Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I

authored by: Mariano Perales, Gustavo Parisi, María Silvina Fornasari, Alejandro Colaneri, Fernando Villarreal, Nahuel González-Schain, Julián Echave, Diego Gómez-Casati, Hans Peter Braun, Alejandro Araya, Eduardo Zabaleta
Abstract: We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtγCAL1 and AtγCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtγCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtγCA and AtγCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtγCAs. All plant species analyzed contain both AtγCA and AtγCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtγCAL sequences show a deviation of functionally important active site residues with respect to γCAs but could form active interfaces in the interaction with AtγCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.
Organisation(s): Institute of Plant Genetics
External Organisation(s): CONICET
Universidad Nacional de Quilmes
Centre national de la recherche scientifique (CNRS)
Universidad Nacional de Mar del Plata
Type: Article
Journal: Plant molecular biology
Volume: 56
Pages: 947-957
No. of pages: 11
ISSN: 0167-4412
Publication date: 07.04.2005
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Agronomy and Crop Science, Genetics, Plant Science
Electronic version(s): https://doi.org/10.1007/s11103-004-6324-z (Access: Unknown)