Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I

verfasst von

Mariano Perales, Gustavo Parisi, María Silvina Fornasari, Alejandro Colaneri, Fernando Villarreal, Nahuel González-Schain, Julián Echave, Diego Gómez-Casati, Hans Peter Braun, Alejandro Araya, Eduardo Zabaleta

Abstract

We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtγCAL1 and AtγCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtγCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtγCA and AtγCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtγCAs. All plant species analyzed contain both AtγCA and AtγCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtγCAL sequences show a deviation of functionally important active site residues with respect to γCAs but could form active interfaces in the interaction with AtγCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.

Organisationseinheit(en)

Institut für Pflanzengenetik

Externe Organisation(en)

CONICET
Universidad Nacional de Quilmes
Centre national de la recherche scientifique (CNRS)
Universidad Nacional de Mar del Plata

Typ

Artikel

Journal

Plant molecular biology

Band

56

Seiten

947-957

Anzahl der Seiten

11

ISSN

0167-4412

Publikationsdatum

07.04.2005

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Agronomie und Nutzpflanzenwissenschaften, Genetik, Pflanzenkunde

Elektronische Version(en)

https://doi.org/10.1007/s11103-004-6324-z (Zugang: Unbekannt)