DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method
- authored by
- Christina Rode, Traud Winkelmann, Hans Peter Braun, Frank Colditz
- Abstract
Two-dimensional difference gel electrophoresis is an invaluable technique for the analysis of plant proteomes. However, preparation of protein fractions from plant tissues is challenging due to the special features of plant cells: a robust cell wall, large vacuoles which often contain high concentrations of organic acids and a broad range of secondary metabolites like phenolic compounds and pigments. Therefore, protein preparation for difference gel electrophoresis (DIGE) analyses has to be adapted. Here, we describe both a phenolic protein extraction method for plant tissues and an adapted protocol for DIGE labeling of the generated fractions.
- Organisation(s)
-
Woody Plant and Propagation Physiology Section
Institute of Plant Genetics
- Type
- Contribution to book/anthology
- Pages
- 335-342
- No. of pages
- 8
- Publication date
- 12.01.2012
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Molecular Biology, Genetics
- Electronic version(s)
-
https://doi.org/10.1007/978-1-61779-573-2_23 (Access:
Unknown)
