DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method

verfasst von

Christina Rode, Traud Winkelmann, Hans Peter Braun, Frank Colditz

Abstract

Two-dimensional difference gel electrophoresis is an invaluable technique for the analysis of plant proteomes. However, preparation of protein fractions from plant tissues is challenging due to the special features of plant cells: a robust cell wall, large vacuoles which often contain high concentrations of organic acids and a broad range of secondary metabolites like phenolic compounds and pigments. Therefore, protein preparation for difference gel electrophoresis (DIGE) analyses has to be adapted. Here, we describe both a phenolic protein extraction method for plant tissues and an adapted protocol for DIGE labeling of the generated fractions.

Organisationseinheit(en)

Abteilung Gehölz- und Vermehrungsphysiologie
Institut für Pflanzengenetik

Typ

Beitrag in Buch/Sammelwerk

Seiten

335-342

Anzahl der Seiten

8

Publikationsdatum

12.01.2012

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Molekularbiologie, Genetik

Elektronische Version(en)

https://doi.org/10.1007/978-1-61779-573-2_23 (Zugang: Unbekannt)