The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont

authored by

E. Tisserant, A. Kohler, P. Dozolme-Seddas, R. Balestrini, K. Benabdellah, A. Colard, D. Croll, C. da Silva, S. K. Gomez, R. Koul, N. Ferrol, V. Fiorilli, D. Formey, P. H. Franken, N. Helber, M. Hijri, L. Lanfranco, E. Lindquist, Y. Liu, M. Malbreil, E. Morin, J. Poulain, H. Shapiro, D. van Tuinen, A. Waschke, C. Azcón-Aguilar, G. Bécard, P. Bonfante, M. J. Harrison, H. Küster, P. Lammers, U. Paszkowski, N. Requena, S. A. Rensing, C. Roux, I. R. Sanders, Y. Shachar-Hill, G. Tuskan, J. P.W. Young, V. Gianinazzi-Pearson, F. Martin

Abstract

• The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. • We generated a set of 25906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. • We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G. intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G. intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. • Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.

Organisation(s)

Section Plant Genomics
Institute of Plant Genetics

External Organisation(s)

Institut national de la recherche agronomique (INRA)
University of Burgundy
University of Turin
Spanish National Research Council (CSIC)
University of Lausanne (UNIL)
ETH Zurich
French Alternative Energies and Atomic Energy Commission (CEA)
Cornell University
New Mexico State University
Universite de Toulouse
Leibniz Institute of Vegetable and Ornamental Crops (IGZ)
Karlsruhe Institute of Technology (KIT)
University of Montreal
U.S. Department of Energy
University of Freiburg
Michigan State University (MSU)
Oak Ridge National Laboratory
Univ. York, Dep. Comput. Sci., Non-Stand. Comput. Group

Type

Article

Journal

New Phytologist

Volume

193

Pages

755-769

No. of pages

15

ISSN

0028-646X

Publication date

01.02.2012

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Plant Science

Electronic version(s)

https://doi.org/10.1111/j.1469-8137.2011.03948.x (Access: Unknown)