The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont

verfasst von

E. Tisserant, A. Kohler, P. Dozolme-Seddas, R. Balestrini, K. Benabdellah, A. Colard, D. Croll, C. da Silva, S. K. Gomez, R. Koul, N. Ferrol, V. Fiorilli, D. Formey, P. H. Franken, N. Helber, M. Hijri, L. Lanfranco, E. Lindquist, Y. Liu, M. Malbreil, E. Morin, J. Poulain, H. Shapiro, D. van Tuinen, A. Waschke, C. Azcón-Aguilar, G. Bécard, P. Bonfante, M. J. Harrison, H. Küster, P. Lammers, U. Paszkowski, N. Requena, S. A. Rensing, C. Roux, I. R. Sanders, Y. Shachar-Hill, G. Tuskan, J. P.W. Young, V. Gianinazzi-Pearson, F. Martin

Abstract

• The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. • We generated a set of 25906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. • We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G. intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G. intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. • Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.

Organisationseinheit(en)

Abteilung Pflanzengenomforschung
Institut für Pflanzengenetik

Externe Organisation(en)

Institut national de la recherche agronomique (INRA)
Universite de Bourgogne
Università di Torino
Spanish National Research Council (CSIC)
Université de Lausanne (UNIL)
ETH Zürich
Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Cornell University
New Mexico State University
Université de Toulouse
Leibniz-Institut für Gemüse- und Zierpflanzenbau Großbeeren (IGZ) e.V.
Karlsruher Institut für Technologie (KIT)
Université de Montréal
U.S. Department of Energy
Albert-Ludwigs-Universität Freiburg
Michigan State University (MSU)
Oak Ridge National Laboratory
University of York

Typ

Artikel

Journal

New Phytologist

Band

193

Seiten

755-769

Anzahl der Seiten

15

ISSN

0028-646X

Publikationsdatum

01.02.2012

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Physiologie, Pflanzenkunde

Elektronische Version(en)

https://doi.org/10.1111/j.1469-8137.2011.03948.x (Zugang: Unbekannt)