Variable DNA content of Cyclamen persicum regenerated via somatic embryogenesis

Rethinking the concept of long-term callus and suspension cultures

authored by

Thomas Borchert, Jörg Fuchs, Traud Winkelmann, Annette Hohe

Abstract

By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation for the absolute DNA content in the range of 3.17 pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes. Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework of future application of in vitro propagation techniques based on regeneration from fastly growing undifferentiated cells like long-term callus and suspension cultures.

Organisation(s)

Research section Reproduction and Development

External Organisation(s)

Technische Hochschule Mittelhessen University of Applied Sciences
Leibniz Institute of Vegetable and Ornamental Crops (IGZ)
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
University of Applied Sciences Weihenstephan-Triesdorf

Type

Article

Journal

Plant Cell, Tissue and Organ Culture

Volume

90

Pages

255-263

No. of pages

9

ISSN

0167-6857

Publication date

21.07.2007

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Horticulture

Electronic version(s)

https://doi.org/10.1007/s11240-007-9264-x (Access: Closed)