Variable DNA content of Cyclamen persicum regenerated via somatic embryogenesis

Rethinking the concept of long-term callus and suspension cultures

verfasst von

Thomas Borchert, Jörg Fuchs, Traud Winkelmann, Annette Hohe

Abstract

By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation for the absolute DNA content in the range of 3.17 pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes. Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework of future application of in vitro propagation techniques based on regeneration from fastly growing undifferentiated cells like long-term callus and suspension cultures.

Organisationseinheit(en)

Abteilung Reproduktion und Entwicklung

Externe Organisation(en)

Technische Hochschule Mittelhessen
Leibniz-Institut für Gemüse- und Zierpflanzenbau Großbeeren (IGZ) e.V.
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
Hochschule Weihenstephan-Triesdorf

Typ

Artikel

Journal

Plant Cell, Tissue and Organ Culture

Band

90

Seiten

255-263

Anzahl der Seiten

9

ISSN

0167-6857

Publikationsdatum

21.07.2007

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Gartenbau

Elektronische Version(en)

https://doi.org/10.1007/s11240-007-9264-x (Zugang: Geschlossen)