The ethylene 2 receptor gene as a robust molecular marker for intergeneric somatic hybrids between Petunia and Calibrachoa

authored by

C. Rode, T. Winkelmann, L. Meyer, T. Debener

Abstract

A 600 bp fragment of the ethylene-receptor gene 2 (ETR2) was sequenced in Petunia and Calibrachoa genotypes and in putative intergeneric somatic hybrids. In total, 16 genus-specific single base pair polymorphisms (SNPs) were detected. These were used to construct, genus specific restriction maps and a Cleaved Amplified Polymorphic Sequence marker with specific 400 bp fragments for Petunia and 550 bp for Calibrachoa. This diagnostic and robust marker is suitable to distinguish Petunia and Calibrachoa and is a valuable tool for the identification of intergeneric somatic hybrids which are currently developed in model breeding programmes. Based on sequence data for the ETR2 fragment, phylogenetic analyses were conducted. Neighbour-Joining trees reveal distinct subgroups of ETR2 of each genus, therefore supporting recent results of molecular phylogenetic studies on Petunia and Calibrachoa. Sequences of putative hybrids are represented in both subgroups, confirming their hybrid status.

Organisation(s)

Woody Plant and Propagation Physiology Section
Section Molecular Plant Breeding

Type

Article

Journal

PLANT BREEDING

Volume

129

Pages

448-453

No. of pages

6

ISSN

0179-9541

Publication date

08.2010

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Agronomy and Crop Science, Genetics, Plant Science

Electronic version(s)

https://doi.org/10.1111/j.1439-0523.2009.01699.x (Access: Unknown)