Comparative sequence analysis of some Rdrl resistance genes from Rosa multiflora

authored by

Aneela Yasmin, Akhtar A. Jalbani, Thomas Debener

Abstract

Rdrl is the first monogenic dominant resistance gene discovered in genus rosa. This locus is active against a fungal disease, black spot, of roses. Rdrl locus consists of nine genes named as muRdrlA to muRdrll. Functional and molecular characterization of this locus, revealed the presence of one gene, muRdrlH, active against Dort E4, race 6 of Diplocarpon rosae. In the present study we aimed to analyze the protein sequences of three members of this Rdrl gene family for their homologies, important domains, conserved regions and selection pressure to get insights of their functionality based on protein sequence. The protein sequences of one active gene, muRdrlH and two inactive genes, mu Rdr 1C and muRdrlG, were deduced and dissected using internet based bioinformatics tools. All three proteins belong to the T1R-NBS-LRR family of resistance genes. Although these proteins carry all necessary domains and conserved regions necessary for the functionality ofTIR-NBS-LRR proteins, LRR region of the proteins, found under selection pressure, indicated its role in effector identification.

Organisation(s)

Section Molecular Plant Breeding

External Organisation(s)

Sindh Agriculture University (SAU)

Type

Article

Journal

Proceedings of the Pakistan Academy of Sciences

Volume

49

Pages

259-267

No. of pages

9

ISSN

0377-2969

Publication date

12.2012

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Social Sciences(all)