Regeneration of different Cyclamen species via somatic embryogenesis from callus, suspension cultures and protoplasts
- authored by
- Anika Nadja Sabine Prange, Melanie Bartsch, Margrethe Serek, Traud Winkelmann
- Abstract
The present study is the first report of the establishment of embryogenic callus cultures from seedling tissue, the regeneration of plants via somatic embryogenesis and the development of a regeneration system from protoplast to plant, using three wild species of Cyclamen, Cyclamen graecum Link, Cyclamen mirabile Hildebrand, Cyclamen trochopteranthum Schwarz (syn. Cyclamen alpinum hort. Dammann ex Sprenger). The ability to form embryogenic callus and to regenerate via somatic embryogenesis was strongly genotype-dependent for each species. From 0.5 g callus, up to 1461 somatic embryos were formed in the case of C. mirabile. Culture media with different concentrations of plant growth regulators, CaCl2 and activated charcoal significantly influenced embryo formation in this species. Up to 1.4 × 106 protoplasts were isolated from 1 g of C. graecum cell suspension. Diverse growth responses of the protoplasts in two embedding agents, agarose and alginate, were observed for the different Cyclamen species. These specific growth characteristics could be used as a selection marker for future fusion experiments. From both protoplast culture systems, somatic embryos were regenerated, grown to plantlets and acclimatised to greenhouse conditions.
- Organisation(s)
-
Research section Reproduction and Development
- Type
- Article
- Journal
- Scientia horticulturae
- Volume
- 125
- Pages
- 442-450
- No. of pages
- 9
- ISSN
- 0304-4238
- Publication date
- 28.06.2010
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Horticulture
- Electronic version(s)
-
https://doi.org/10.1016/j.scienta.2010.04.018 (Access:
Closed)
