Proteomic and physiological responses of the halophyte Cakile maritima to moderate salinity at the germinative and vegetative stages

authored by

Ahmed Debez, Hans Peter Braun, Andreas Pich, Wael Taamalli, Hans Werner Koyro, Chedly Abdelly, Bernhard Huchzermeyer

Abstract

Responses of the halophyte Cakile maritima to moderate salinity were addressed at germination and vegetative stages by bringing together proteomics and eco-physiological approaches. 75. mM NaCl-salinity delayed significantly the germination process and decreased slightly the seed germination percentage compared to salt-free conditions. Monitoring the proteome profile between 0. h and 120. h after seed sowing revealed a delay in the degradation of seed storage proteins when germination took place under salinity, which may explain the slower germination rate observed. Of the sixty-seven proteins identified by mass spectrometry, several proteins involved in glycolysis, amino acid metabolism, photosynthesis, and protein folding showed significantly increased abundance during germination. This pattern was less pronounced under salinity. At the vegetative stage, 100. mM NaCl-salinity stimulated significantly the plant growth, which was sustained by enhanced leaf expansion, water content, and photosynthetic activity. Comparative proteome analyses of leaf tissue revealed 44 proteins with different abundance changes, most of which being involved in energy metabolism. A specific set of proteins predominantly involved in photosynthesis and respiration showed significantly higher abundance in salt-treated plants. Altogether, combining proteomics with eco-physiological tools provides valuable information, which contributes to improve our understanding in the salt-response of this halophyte during its life cycle.

Organisation(s)

Institute of Plant Genetics

External Organisation(s)

Center of Biotechnology of Borj Cedria (CBBC)
Hannover Medical School (MHH)
Justus Liebig University Giessen

Type

Article

Journal

Journal of proteomics

Volume

75

Pages

5667-5694

No. of pages

28

ISSN

1874-3919

Publication date

29.08.2012

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biophysics, Biochemistry

Sustainable Development Goals

SDG 15 - Life on Land

Electronic version(s)

https://doi.org/10.1016/j.jprot.2012.08.012 (Access: Unknown)