Assignment of the Auxin Binding Abilities of ABP₄₄ in Gel

authored by

Thomas Reinard, Heike Achmus, Antje Walther, Ursula Rescher, Dieter Klämbt, Hans Jörg Jacobsen

Abstract

Several approaches were successfully performed to directly assign and characterize auxin binding of ABP₄₄ in gel. The 44 kDa high affinity auxin binding protein ABP₄₄ from pea was tested for its ability to bind 5-azido-[7-³H]-IAA in photoaffinity labeling experiments. Competition experiments with several auxin analogues confirm data published previously (Reinard and Jacobsen 1995). Critical reflections of the limitations of 'the method are also discussed. Immunostaining using the antibody D16 (Napier and Venis 1992), which is directed against the putative binding site of ABP1, revealed that ABP₄₄'s auxin binding site is at least partially related to the corresponding site of ABP1. Nevertheless, both proteins do not share any further immunological relationships. Our results with D16 recommend a careful reconsideration of data published by other authors. Furthermore, a 80 kDa, dimeric glutathione dependent formaldehyde dehydrogenase (FDH) from mung bean, described recently, was found to be different from ABP₄₄. In contrast to the described FDH, ABP₄₄ exhibited no FDH activity.

Organisation(s)

Institute of Plant Genetics

External Organisation(s)

University of Bonn

Type

Article

Journal

Plant and Cell Physiology

Volume

39

Pages

874-878

No. of pages

5

ISSN

0032-0781

Publication date

01.08.1998

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Plant Science, Cell Biology

Electronic version(s)

https://doi.org/10.1093/oxfordjournals.pcp.a029447 (Access: Open)