Blue-native PAGE in plants

A tool in analysis of protein-protein interactions

authored by

Holger Eubel, Hans Peter Braun, A. Harvey Millar

Abstract

Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.

Organisation(s)

Institute of Plant Genetics

External Organisation(s)

University of Western Australia

Type

Review article

Journal

PLANT METHODS

Volume

1

ISSN

1746-4811

Publication date

16.11.2005

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Genetics, Plant Science

Electronic version(s)

https://doi.org/10.1186/1746-4811-1-11 (Access: Open)