Biochemical properties of a novel cell wall protein associated with elongation growth in higher plants

authored by

Thomas Reinard, Stefanie Sprunck, Sandra Altherr, Hans Jörg Jacobsen

Abstract

A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.

Organisation(s)

Institute of Plant Genetics

Type

Article

Journal

Journal of experimental botany

Volume

45

Pages

1593-1601

No. of pages

9

ISSN

0022-0957

Publication date

01.11.1994

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Plant Science

Electronic version(s)

https://doi.org/10.1093/jxb/45.11.1593 (Access: Closed)