Micropropagation through somatic embryogenesis of Cyclamen persicum mill. Genotypes for cut flower production - feasibility study

authored by

Traud Winkelmann, Agnieszka Ilczuk, Stephan Wartenberg

Abstract

The regeneration of Cyclamen persicum through somatic embryogenesis (SE) has been described by several groups for a wide spectrum of genotypes. Here, we studied this regeneration system for the first time with 30 genotypes which are valued as cut flowers. The aim of this study was to analyse the efficiency of the micropropagation through SE, to investigate the genetic fidelity and performance of the regenerants and to estimate production costs of young plants. The genotype significantly influenced the frequency of callus induction that ranged from 8 to 80% and also the frequency of calluses with differentiation of somatic embryos that ranged from 0 to 92%. Only three of the 30 genotypes were not able to regenerate somatic embryos, while plants were obtained from the remaining 27 genotypes. In total, 2,783 regenerants were transferred to the greenhouse, 2,003 (72%) of which could be acclimatized. Some genotypes were extremely uniform in terms of flower colour and flower shapes, whereas in others somaclonal variation was detected. Estimation of micropropagation cost was 2-3 € per acclimatized young plant at our research facility. If genotypes with high regeneration efficiencies are selected, plant production costs could be reduced to 0.56-0.80 € or even more.

Organisation(s)

Research section Reproduction and Development

External Organisation(s)

Warsaw Agricultural University
Saxon State Office for Environment, Agriculture and Geology (LfULG)

Type

Article

Journal

Propagation of Ornamental Plants

Volume

10

Pages

237-245

No. of pages

9

ISSN

1311-9109

Publication date

2010

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Forestry, Plant Science