Pathogenesis and stress related, as well as metabolic proteins are regulated in tomato stems infected with Ralstonia solanacearum

authored by

Diwakar Dahal, Dimitri Heintz, Alain Van Dorsselaer, Hans Peter Braun, Kerstin Wydra

Abstract

A comparative proteome analysis was initiated to systematically investigate the physiological response of tomato (Solanum lycopersicum) to infection with Ralstonia solanacearum, causal agent of bacterial wilt. Plants of the susceptible tomato recombinant inbred line NHG3 and the resistant NHG13 were either infected or not infected with R. solanacearum and subsequently used for proteome analysis. Two-dimensional isoelectric focussing/sodium dodecyl-sulphate polyacrylamide gel electrophoresis (2-D IEF/SDS-PAGE) allowed the separation of about 650-690 protein spots per analysis. Twelve proteins were of differential abundance in susceptible plants in response to bacterial infection, while no differences were observed in the resistant genotype. LC-MS/MS analysis of these spots revealed 12 proteins, six of which were annotated as plant and six as bacterial proteins. Among the plant proteins, two represent pathogenesis related (PR) proteins, one stress response protein, one enzyme of carbohydrate and energy metabolism, and one hypothetical protein. A constitutive difference between resistant and susceptible lines was not found.

Organisation(s)

Institute of Plant Genetics

External Organisation(s)

Centre national de la recherche scientifique (CNRS)

Type

Article

Journal

Plant physiology and biochemistry

Volume

47

Pages

838-846

No. of pages

9

ISSN

0981-9428

Publication date

19.05.2009

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Genetics, Plant Science

Electronic version(s)

https://doi.org/10.1016/j.plaphy.2009.05.001 (Access: Unknown)