Proteomic and phosphoproteomic analysis of polyethylene glycol-induced osmotic stress in root tips of common bean (Phaseolus vulgaris L.)

authored by

Zhong Bao Yang, Dejene Eticha, Hendrik Führs, Dimitri Heintz, Daniel Ayoub, Alain Van Dorsselaer, Barbara Schlingmann, Idupulapati Madhusudana Rao, Hans Peter Braun, Walter Johannes Horst

Abstract

Previous studies have shown that polyethylene glycol (PEG)-induced osmotic stress (OS) reduces cell-wall (CW) porosity and limits aluminium (Al) uptake by root tips of common bean (Phaseolus vulgaris L.). A subsequent transcriptomic study suggested that genes related to CW processes are involved in adjustment to OS. In this study, a proteomic and phosphoproteomic approach was applied to identify OS-induced protein regulation to further improve our understanding of how OS affects Al accumulation. Analysis of total soluble proteins in root tips indicated that, in total, 22 proteins were differentially regulated by OS; these proteins were functionally categorized. Seventy-seven per- cent of the total expressed proteins were involved in metabolic pathways, particularly of carbohydrate and amino acid metabolism. An analysis of the apoplastic proteome revealed that OS reduced the level of five proteins and increased that of seven proteins. Investigation of the total soluble phosphoproteome suggested that dehydrin responded to OS with an enhanced phosphorylation state without a change in abundance. A cellular immunolocalization analysis indicated that dehydrin was localized mainly in the CW. This suggests that dehydrin may play a major protective role in the OS-induced physical breakdown of the CW structure and thus maintenance of the reversibility of CW extensibility during recovery from OS. The proteomic and phosphoproteomic analyses provided novel insights into the complex mechanisms of OS-induced reduction of Al accumulation in the root tips of common bean and highlight a key role for modification of CW structure.

Organisation(s)

Institute of Plant Genetics
Institute of Plant Nutrition

External Organisation(s)

Shandong University
Yara GmbH
K+S KALI GmbH
University of Strasbourg
International Center for Tropical Agriculture (CIAT)

Type

Article

Journal

Journal of experimental botany

Volume

64

Pages

5569-5586

No. of pages

18

ISSN

0022-0957

Publication date

11.10.2013

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Plant Science

Electronic version(s)

https://doi.org/10.1093/jxb/ert328 (Access: Open)