The carbonic anhydrase domain of plant mitochondrial complex I

authored by

Steffanie Fromm, Jennifer Senkler, Eduardo Zabaleta, Christoph Peterhänsel, Hans Peter Braun

Abstract

The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling gamma-type carbonic anhydrases. The Arabidopsis genome codes for five complex I-integrated gamma-type carbonic anhydrases (γCA1, γCA2, γCA3, γCAL1, γCAL2), but only three copies of this group of proteins form an individual extra domain. Biochemical analyses revealed that the domain is composed of one copy of either γCAL1 or γCAL2 plus two copies of the γCA1/γCA2 proteins. Thus, the carbonic anhydrase domain can have six distinct subunit configurations. Single and double mutants with respect to the γCA/γCAL proteins were employed to genetically dissect the function of the domain. New insights into complex I biology in plants will be reviewed and discussed.

Organisation(s)

Section Plant Molecular Biology and Plant Proteomics
Institute of Botany
Institute of Plant Genetics

External Organisation(s)

Universidad Nacional de Mar del Plata

Type

Review article

Journal

Physiologia plantarum

Volume

157

Pages

289-296

No. of pages

8

ISSN

0031-9317

Publication date

21.06.2016

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Genetics, Plant Science, Cell Biology

Electronic version(s)

https://doi.org/10.15488/11655 (Access: Open)
https://doi.org/10.1111/ppl.12424 (Access: Closed)