The production of a genus-specific recombinant antibody (scFv) using a recombinant potyvirus protease

authored by

Thomas Reinard, Edgar Maiß, Michael Hust

Abstract

A single chain variable fragment antibody (scFv; anti-NIa scFv102) was selected from a synthetic human antibody library by using a NIa protease of Plum pox virus (PPV) as an antigen, which was expressed in bacteria. The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. The NIa protein was detected with anti-NIa scFv102 after expression in Escherichia coli cells as well as from PPV-infected Nicotiana benthamiana plants. Furthermore, the scFv102 has the ability to identify not only PPV from infected plants but also can detect other infections with members of the potyviruses. Nineteen different potyviruses were recognized by the scFv102 in various infected plants tested through dot blot assays. Therefore, the antibody scFv102 has the potential of becoming a general tool to detect potyvirus infections in different plant species. ?? 2002 Published by Elsevier Science B.V.

Organisation(s)

Section Plant Biotechnology
Phytomedicine Section

External Organisation(s)

Technische Universität Braunschweig

Type

Article

Journal

Journal of Virological Methods

Volume

106

Pages

225-233

ISSN

0166-0934

Publication date

2002

Publication status

Published

Peer reviewed

Yes

Electronic version(s)

https://doi.org/10.1016/S0166-0934(02)00166-0 (Access: Restricted)