Morphological characterization of plants regenerated from PROTOPLASTS of Cyclamen persicum Mill

verfasst von

Traud Winkelmann, Anika Nadja Sabine Prange, Janine Specht, Margrethe Serek

Abstract

A protoplast to plant regeneration system will open the way for somatic hybridization in cyclamen (Cyclamen persicum Mill.). A protocol for efficient plant regeneration from protoplasts isolated from embryogenic suspensions has been developed in cyclamen for the first time, and then successfully applied to different genotypes. Enzymatic digestion in 2.0% cellulose R10 and 0.5% macerozyme R10 resulted in yields between 1.0 and 5.0 × 10⁵ protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. Cell division was recorded for all eleven tested genotypes in frequencies of up to 12% and 18% after seven and 14 days, respectively. Plant regeneration via somatic embryogenesis succeeded in nine out of the 11 genotypes under investigation. Protoplast-derived plants from eight genotypes have been successfully transferred to soil. Somaclonal variation was detected in terms of ploidy levels of regenerants, malformed flowers, and formation of multiple vegetative meristems with delayed flowering. However, its degree depended on the genotype and the time period between isolation of protoplasts and plant regeneration.

Organisationseinheit(en)

Abteilung Reproduktion und Entwicklung

Externe Organisation(en)

Hochschule Weihenstephan-Triesdorf

Typ

Artikel

Journal

Propagation of Ornamental Plants

Band

8

Seiten

9-12

Anzahl der Seiten

4

ISSN

1311-9109

Publikationsdatum

03.2008

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Forstwissenschaften, Pflanzenkunde