Comparative Analyses of Protein Complexes by Blue Native DIGE

verfasst von
Katrin Peters, Hans Peter Braun
Abstract

Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.

Organisationseinheit(en)
Institut für Pflanzengenetik
Typ
Beitrag in Buch/Sammelwerk
Seiten
145-154
Anzahl der Seiten
10
Publikationsdatum
12.01.2012
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ASJC Scopus Sachgebiete
Molekularbiologie, Genetik
Elektronische Version(en)
https://doi.org/10.1007/978-1-61779-573-2_10 (Zugang: Unbekannt)