Comparative Analyses of Protein Complexes by Blue Native DIGE
- verfasst von
- Katrin Peters, Hans Peter Braun
- Abstract
Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.
- Organisationseinheit(en)
-
Institut für Pflanzengenetik
- Typ
- Beitrag in Buch/Sammelwerk
- Seiten
- 145-154
- Anzahl der Seiten
- 10
- Publikationsdatum
- 12.01.2012
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Molekularbiologie, Genetik
- Elektronische Version(en)
-
https://doi.org/10.1007/978-1-61779-573-2_10 (Zugang:
Unbekannt)