Complexome Profiling Reveals Association of PPR Proteins with Ribosomes in the Mitochondria of Plants

verfasst von

Nils Rugen, Henryk Straube, Linda E. Franken, Hans Peter Braun, Holger Eubel

Abstract

Mitochondrial transcripts are subject to a wealth of processing mechanisms including cis- and trans-splicing events, as well as base modifications (RNA editing). HUndreds of proteins are required for these processes in plant mitochondria, many of which belong to the pentatricopeptide repeat (PPR) protein superfamily. The structure, localization, and function of these proteins is only poorly Understood. Here we present evidence that several PPR proteins are boUnd to mitoribosomes in plants. A novel complexome profiling strategy in combination with chemical crosslinking has been employed to systematically define the protein constituents of the large and the small ribosomal subunits in the mitochondria of plants. We identified more than 80 ribosomal proteins, which include several PPR proteins and other non-conventional ribosomal proteins. These findings reveal a potential coupling of transcriptional and translational events in the mitochondria of plants. Furthermore, the data indicate an extremely high molecular mass of the “small” subunit, even exceeding that of the “large” subunit.

Organisationseinheit(en)

Institut für Pflanzengenetik

Externe Organisation(en)

Heinrich-Pette-Institut Leibniz-Institut für Experimentelle Virologie (HPI)

Typ

Artikel

Journal

Molecular and Cellular Proteomics

Band

18

Seiten

1345-1362

Anzahl der Seiten

18

ISSN

1535-9476

Publikationsdatum

07.2019

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Analytische Chemie, Biochemie, Molekularbiologie

Elektronische Version(en)

https://doi.org/10.1074/mcp.RA119.001396 (Zugang: Offen)
https://doi.org/10.15488/11680 (Zugang: Offen)
https://doi.org/10.15488/12222 (Zugang: Offen)
https://doi.org/10.1074/mcp.AAC119.001674 (Zugang: Offen)