Mating biology, nuclear DNA content and genetic diversity in spider plant (Cleome gynandra) germplasm from various African countries

verfasst von

Emmanuel O. Omondi, Thomas Debener, Marcus Linde, Mary Abukutsa-Onyango, Fekadu F. Dinssa, Traud Winkelmann

Abstract

Cleome gynandra (L.) Briq. is an African leafy vegetable with a potential to improve food security and micronutrient deficiencies. Cytological traits, breeding biology and genetic diversity of 30 selected entries of C. gynandra from six African countries were investigated. The entries consisted of advanced lines, gene bank accessions and farmers’ cultivars. Our study revealed chromosome numbers of 2n = 34 in root tip metaphase cells from one entry. The 30 entries were found to be diploid with genome sizes ranging from 2.31 to 2.45 pg/2C. Hand pollination experiments were carried out to assess self-incompatibility within the entries and revealed that they are self- and cross-compatible. For genetic diversity studies within and among the entries, the pooled data of 499 polymorphic bands from 11 amplified fragment length polymorphism primer combinations and nine simple sequence repeat markers were used. The genetic distance among the entries ranged from 0.13 to 0.77. In a principal coordinate analysis, the farmers’ cultivars formed a cluster separate from the advanced lines and the gene bank entries, and the latter were not well resolved.

Organisationseinheit(en)

Institut für Pflanzengenetik
Institut für Gartenbauliche Produktionssysteme

Externe Organisation(en)

Jomo Kenyatta University of Agriculture and Technology
AVRDC - The World Vegetable Center

Typ

Artikel

Journal

Plant Breeding

Band

136

Seiten

578-589

Anzahl der Seiten

12

ISSN

0179-9541

Publikationsdatum

08.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Agronomie und Nutzpflanzenwissenschaften, Genetik, Pflanzenkunde

Ziele für nachhaltige Entwicklung

SDG 2 – Kein Hunger

Elektronische Version(en)

https://doi.org/10.1111/pbr.12485 (Zugang: Geschlossen)