Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III
- verfasst von
- Natalia V. Dudkina, Holger Eubel, Wilko Keegstra, Egbert J. Boekema, Hans Peter Braun
- Abstract
Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes: NADH-ubiquinone oxidoreductase (commonly known as complex I), succinate-ubiquinone oxidoreductase (complex II), ubiquinol-cytochrome c oxidoreductase (cytochrome bc1 complex or complex III), cytochrome c-O2 oxidoreductase (complex IV), and F 1F0-ATP synthase (complex V). Several lines of evidence have recently suggested that complexes I and III-V might interact to form supercomplexes. However, because of their fragility, the structures of these supercomplexes are still unknown. A stable supercomplex consisting of complex I and dimeric complex III was purified from plant mitochondria. Structural characterization by single-particle EM indicates a specific type of interaction between monomeric complex I and dimeric complex III in a 1:1 ratio. We present a model for how complexes I and III are spatially organized within the I+III 2 supercomplex.
- Organisationseinheit(en)
-
Institut für Pflanzengenetik
- Externe Organisation(en)
-
Reichsuniversität Groningen
- Typ
- Artikel
- Journal
- Proceedings of the National Academy of Sciences of the United States of America
- Band
- 102
- Seiten
- 3225-3229
- Anzahl der Seiten
- 5
- ISSN
- 0027-8424
- Publikationsdatum
- 03.2005
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Allgemein
- Elektronische Version(en)
-
https://doi.org/10.1073/pnas.0408870102 (Zugang:
Offen)