Agrobacterium tumefaciens-mediated transformation of oncidium and odontoglossum orchid species with the ethylene receptor mutant gene etr1-1

verfasst von

Barbara Raffeiner, Margrethe Serek, Traud Winkelmann

Abstract

Oncidium and Odontoglosum orchid species have reduced display lives and are thus commercially less important than Phalaenopsis. One approach to prolonging display life permanently is to transform Oncidium and Odontoglossum with the ethylene receptor mutant gene etr1-1 from Arabidopsis under control of a flower specific promoter; this should reduce their sensitivity to exogenous ethylene. To achieve this it will be necessary to establish an efficient regeneration protocol using somatic embryogenesis and a routine Agrobacterium tumefaciens-mediated transformation procedure. Protocorm-like bodies (PLBs) of both orchid genera were regenerated from leaf tip explants. Leaf tips and PLBs, cultured in liquid and solid media, were compared as targets for genetic transformation. No transgenic shoots were obtained from leaf tips, while PLBs of Oncidium and Odontoglossum cultured on solid medium were successfully transformed with an expression vector containing nptII and gus genes driven by the cauliflower mosaic virus (CaMV) 35S promoter. Applying the A. tumefaciens strain EHA 105, transformation efficiencies of 1.3-2.7% were achieved for the investigated genotypes. Transformation with etr1-1 gene was achieved subsequently. Oncidium 'Sweet Sugar' has been successfully transformed and validated by PCR and Southern analysis.

Organisationseinheit(en)

Abteilung Reproduktion und Entwicklung

Externe Organisation(en)

Hochschule Weihenstephan-Triesdorf

Typ

Artikel

Journal

Plant Cell, Tissue and Organ Culture

Band

98

Seiten

125-134

Anzahl der Seiten

10

ISSN

0167-6857

Publikationsdatum

13.06.2009

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Gartenbau

Elektronische Version(en)

https://doi.org/10.1007/s11240-009-9545-7 (Zugang: Geschlossen)