Functional annotation of 2D protein maps

The GelMap portal

verfasst von

Michael Senkler, Hans Peter Braun

Abstract

In classical proteome analyses, final experimental data are (a) images of 2D protein separations obtained by gel electrophoresis and (b) corresponding lists of proteins which were identified by mass spectrometry (MS). For data annotation, software tools were developed which allow the linking of protein identity data directly to 2D gels ("clickable gels"). GelMap is a new online software tool to annotate 2D protein maps. It allows (i) functional annotation of all identified proteins according to biological categories defined by the user, e.g., subcellular localization, metabolic pathway, or assignment to a protein complex and (ii) annotation of several proteins per analyzed protein "spot" according to MS primary data. Options to differentially display proteins of functional categories offer new opportunities for data evaluation. For instance, if used for the annotation of 2D Blue native/SDS gels, GelMap allows the identification of protein complexes of low abundance. A web portal has been established for presentation and evaluation of protein identity data related to 2D gels and is freely accessible at www.gelmap.de.

Organisationseinheit(en)

Abteilung Pflanzenmolekularbiologie und Pflanzenproteomik
Institut für Pflanzengenetik

Typ

Übersichtsarbeit

Journal

Frontiers in Plant Science

Band

ISSN

1664-462X

Publikationsdatum

14.05.2012

Publikationsstatus

Veröffentlicht

Peer-reviewed

ASJC Scopus Sachgebiete

Pflanzenkunde

Elektronische Version(en)

https://doi.org/10.3389/fpls.2012.00087 (Zugang: Offen)