Selective Enrichment of Methylococcaceae versus Methylocystaceae Methanotrophs via Control of Methane Feeding Schemes
- verfasst von
- Ju Yong Lee, Munjeong Choi, Min Joon Song, Daehyun Daniel Kim, Taeho Yun, Jin Chang, Adrian Ho, Jaewook Myung, Sukhwan Yoon
- Abstract
Methanotrophs are crucial in keeping environmental CH4 emissions in check. However, the contributions of different groups of methanotrophs at terrestrial CH4-oxidation hotspots, such as the oxic-anoxic interface of rice paddies, have shown considerable inconsistency across observations. To address the knowledge gap regarding this inconsistency, methanotrophic microbiomes were enriched from paddy soils in well-mixed CH4-fed batch reactors under six different incubation conditions, prepared as combinations of two CH4 mixing ratios (0.5 and 10%) and three supplemented Cu2+ concentrations (0, 2, and 10 μM). Monitoring of temporal community shifts in these cultures revealed a dominance of Methylocystis spp. in all 0.5%-CH4 cultures, while methanotrophs affiliated to Gammaproteobacteria dominated the 10%-CH4 cultures that were less consistent both temporally and across conditions. The shotgun metagenome analyses of the 0.5%-CH4 cultures corroborated the Methylocystis dominance and, interestingly, showed that copper deficiency did not select for mmoXYZ-possessing methanotrophs. Instead, a mbn cluster, accounting for approximately 5% of the Methylocystis population, was identified, suggesting the ecological significance of methanobactin in Cu-deficient methanotrophy. These findings underscore the important role of Methylocystis spp. in mitigating emissions from terrestrial CH4 hotspots and suggest the feasibility of directed enrichment and/or isolation of Methylocystis spp. for utilization in, for example, methanobactin and polyhydroxybutyrate production.
- Organisationseinheit(en)
-
Institut für Mikrobiologie
- Externe Organisation(en)
-
Korea Advanced Institute of Science and Technology (KAIST)
University of California at Berkeley
- Typ
- Artikel
- Journal
- Environmental Science and Technology
- Band
- 58
- Seiten
- 14237-14248
- Anzahl der Seiten
- 12
- ISSN
- 0013-936X
- Publikationsdatum
- 13.08.2024
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Chemie (insg.), Umweltchemie
- Elektronische Version(en)
-
https://doi.org/10.1021/acs.est.4c02655 (Zugang:
Geschlossen)
