3D gel map of Arabidopsis complex I

verfasst von

Katrin Peters, Katharina Belt, Hans Peter Braun

Abstract

Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap www.gelmap.de/arabidopsis-3d-complex-i/ is presented for promoting future complex I research in Arabidopsis thaliana.

Organisationseinheit(en)

Institut für Pflanzengenetik

Typ

Artikel

Journal

Frontiers in Plant Science

Band

4

ISSN

1664-462X

Publikationsdatum

04.06.2013

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Pflanzenkunde

Elektronische Version(en)

https://doi.org/10.3389/fpls.2013.00153 (Zugang: Offen)