Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability

verfasst von
Kathleen Prinz, Stephan Schie, Thomas Debener, Isabell Hensen, Kurt Weising
Abstract

We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.

Organisationseinheit(en)
Institut für Pflanzengenetik
Abteilung Molekulare Pflanzenzüchtung
Externe Organisation(en)
Universität Kassel
Martin-Luther-Universität Halle-Wittenberg
Typ
Artikel
Journal
Molecular ecology resources
Band
9
Seiten
1424-1426
Anzahl der Seiten
3
ISSN
1755-098X
Publikationsdatum
09.2009
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ASJC Scopus Sachgebiete
Biotechnologie, Ökologie, Evolution, Verhaltenswissenschaften und Systematik, Genetik
Elektronische Version(en)
https://doi.org/10.1111/j.1755-0998.2009.02680.x (Zugang: Unbekannt)