Transient gene expression in rose petals via Agrobacterium infiltration

verfasst von

Aneela Yasmin, Thomas Debener

Abstract

The study of gene function in roses is hampered by the low efficiency of transformation systems and the long time span needed for the generation of transgenic plants. For some functional analyses, the transient expression of genes would be an efficient alternative. Based on current protocols for the transient expression of genes via the infiltration of Agrobacterium into plant tissues, we developed a transient expression system for rose petals. We used β-glucuronidase (GUS) as a marker gene to optimize several parameters with effects on GUS expression. The efficiency of expression was found to be dependent on the rose genotype, flower age, position of petals within a flower, Agrobacterium strain and temperature of co-cultivation. The highest GUS expression was recorded in petals of the middle whirls of half-bloomed flowers from cultivars of 'Pariser Charme' and 'Marvel'.

Organisationseinheit(en)

Institut für Pflanzengenetik
Abteilung Molekulare Pflanzenzüchtung

Typ

Artikel

Journal

Plant Cell, Tissue and Organ Culture

Band

102

Seiten

245-250

Anzahl der Seiten

6

ISSN

0167-6857

Publikationsdatum

08.2010

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Gartenbau

Elektronische Version(en)

https://doi.org/10.1007/s11240-010-9728-2 (Zugang: Unbekannt)