Proteomic and histological analyses of endosperm development in Cyclamen persicum as a basis for optimization of somatic embryogenesis

verfasst von

Jenniffer Wamaitha Mwangi, Christina Rode, Frank Colditz, Christin Haase, Hans Peter Braun, Traud Winkelmann

Abstract

The endosperm plays an important role for the development of zygotic embryos, while somatic embryos lack a seed coat and endosperm and often show physiological disorders. This study aims at elucidating the cellular and physiological processes within the endosperm of the ornamental species Cyclamen persicum Mill. Histological analyses were performed from 0 to 11 weeks after pollination (WAP). At 3. WAP, a syncytium was clearly visible with a globular zygotic embryo. From 4. WAP, cellularization of the endosperm, at 5. WAP a small torpedo shaped embryo, and from 7. WAP cell expansion was observed. By 11. WAP the endosperm appeared fully differentiated. Total soluble proteins were extracted from the endosperm at 4, 5, 7, 9 and 11. WAP and resolved using two dimensional isoelectric focussing/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE). A shift from high-molecular-mass proteins to low-molecular-mass proteins during endosperm development was observed. A total of 1137. protein. spots/gel were detected in the three protein fractions extracted at 7, 9 and 11. WAP. Mass spectrometry analysis of the 48 predominant protein spots in endosperm at 7, 9 and 11. WAP resulted in the identification of 62 proteins, ten of which were described for the first time in Cyclamen. Additionally, 186 proteins were identified using the C. persicum embryo proteome reference map. Proteins involved in abscisic acid signalling and oxidative stress responsive proteins were found to be important for seed development in Cyclamen. The new insights into endosperm physiology including storage compounds are discussed.

Organisationseinheit(en)

Institut für Pflanzengenetik
Abteilung Gehölz- und Vermehrungsphysiologie

Typ

Artikel

Journal

Plant science

Band

201-202

Seiten

52-65

Anzahl der Seiten

14

ISSN

0168-9452

Publikationsdatum

27.11.2012

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Genetik, Agronomie und Nutzpflanzenwissenschaften, Pflanzenkunde

Elektronische Version(en)

https://doi.org/10.1016/j.plantsci.2012.11.004 (Zugang: Unbekannt)