Low-SDS Blue native PAGE

verfasst von

Jennifer Klodmann, Dagmar Lewejohann, Hans Peter Braun

Abstract

SDS normally is strictly avoided during Blue native (BN) PAGE because it leads to disassembly of protein complexes and unfolding of proteins. Here, we report a modified BN-PAGE procedure, which is based on low-SDS treatment of biological samples prior to native gel electrophoresis. Using mitochondrial OXPHOS complexes from Arabidopsis as a model system, low SDS concentrations are shown to partially dissect protein complexes in a very defined and reproducible way. If combined with 2-D BN/SDS-PAGE, generated subcomplexes and their subunits can be systematically investigated, allowing insights into the internal architecture of protein complexes. Furthermore, a 3-D BN/low-SDS BN/SDS-PAGE system is introduced to facilitate structural analysis of individual protein complexes without their previous purification.

Organisationseinheit(en)

Abteilung Pflanzenmolekularbiologie und Pflanzenproteomik
Institut für Pflanzengenetik

Typ

Artikel

Journal

PROTEOMICS

Band

11

Seiten

1834-1839

Anzahl der Seiten

6

ISSN

1615-9853

Publikationsdatum

25.02.2011

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biochemie, Molekularbiologie

Elektronische Version(en)

https://doi.org/10.1002/pmic.201000638 (Zugang: Unbekannt)