Tat transport in Escherichia coli requires zwitterionic phosphatidylethanolamine but no specific negatively charged phospholipid

verfasst von

Claudia Rathmann, Amelie S. Schlösser, Jürgen Schiller, Mikhail Bogdanov, Thomas Brüser

Abstract

Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully functional in a clsA/clsB/clsC deletion strain lacking CL, and in a pgsA deletion strain lacking both PG and CL during aerobic growth on complex media. In contrast to early studies that relied on strains with reduced lipid levels, this study therefore demonstrates that PG and CL are dispensable for Tat transport. The lack of these lipids may be compensated by other anionic phospholipids such as phosphatidic acid, CDP-diacylglycerol or N-acyl-PE.

Organisationseinheit(en)

Institut für Mikrobiologie

Externe Organisation(en)

Universität Leipzig
McGovern Medical School

Typ

Artikel

Journal

FEBS letters

Band

591

Seiten

2848-2858

Anzahl der Seiten

11

ISSN

0014-5793

Publikationsdatum

09.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biophysik, Strukturelle Biologie, Biochemie, Molekularbiologie, Genetik, Zellbiologie

Elektronische Version(en)

https://doi.org/10.1002/1873-3468.12794 (Zugang: Offen)